Recombinant Mouse Sema3a protein(Lys26-Phe546), hFc-tagged

• Cat.No.: Sema3a-7021M
• Product Overview: Recombinant Mouse SEMA3A (O08665)(Lys 26-Phe 546) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Lys26-Phe546
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse SEMA3A/Fc is a disulfide-linked homodimer The reduced monomer consists of 781 amino acids and has a predicted molecular mass of 87.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm SEMA3A/Fc monomer is approximately 100 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sema3a sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Mus musculus ]
• Official Symbol: Sema3a
• Synonyms: SEMA3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; semaphorin-3A; sema D; sema III; collapsin-1; semaphorin-D; semaphorin III; SemD; SEMA1; Semad; coll-1; Hsema-I
• Gene ID: 20346
• mRNA Refseq: NM_001243072
• Protein Refseq: NP_001230001