Active Recombinant Mouse Sema3a Protein, Fc-tagged

• Cat.No.: Sema3a-01M
• Product Overview: Recombinant Mouse Semaphorin 3A (Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg618Ala, Lys619Ala, Lys642Ala, Lys643Ala) with a mouse IgG2A (Glu98-Lys330) Fc tag was expressed in chinese hamster ovary cell line.

Specification

• Description: Enables chemorepellent activity; neuropilin binding activity; and semaphorin receptor binding activity. Involved in several processes, including nervous system development; positive regulation of male gonad development; and ventral trunk neural crest cell migration. Acts upstream of or within several processes, including dichotomous subdivision of terminal units involved in salivary gland branching; generation of neurons; and negative regulation of epithelial cell migration. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; embryo mesenchyme; metanephros; and sensory organ. Human ortholog(s) of this gene implicated in hypogonadotropic hypogonadism 16 with or without anosmia. Orthologous to human SEMA3A (semaphorin 3A).
• Source: CHO
• Species: Mouse
• Tag: Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Mouse Semaphorin 3A Fc Chimera causes > 50 % growth cone collapse in the presence 10 ng/mL Recombinant Human beta‑NGF.
• Molecular Mass: 115-120 kDa, reducing conditions
• N-terminal Sequence Analysis: Asn21
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 90 % by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing at least 0.1 % human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature.
• Protein length: Asn21-Lys747

Gene Information

• Gene Name: Sema3a sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Mus musculus (house mouse) ]
• Official Symbol: Sema3a
• Synonyms: Sema3a; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; SemD; SEMA1; Semad; coll-1; Hsema-I; semaphorin-3A; collapsin-1; sema D; sema III; semaphorin III; semaphorin-D
• Gene ID: 20346
• mRNA Refseq: NM_009152
• Protein Refseq: NP_033178
• UniProt ID: O08665