Active Recombinant Human SEMA3A Protein, Met-6-His/Fc-tagged

Cat.No. : SEMA3A-13H
Product Overview : Recombinant Human Semaphorin 3A (Lys26-Val771) (Arg552Ala & Arg555Ala) protein with Met-6-His tag at N-terminus and Human IgG1 (Pro100-Lys330) Fc tag at N-terminal was expressed in Mouse myeloma cell line.
  • Specification
  • Gene Information
  • Related Products
  • Download
Species : Human
Source : NS0
Tag : Fc&His
Protein Length : 26-771 a.a.
Description : This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
Form : Disulfide-linked homodimer
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Rat Neuropilin-1 Fc Chimera is coated at 2 μg/mL, Recombinant Human Semaphorin 3A Fc Chimera binds with an ED50 = 50-300 ng/mL. Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera causes > 50 % growth cone collapse in the presence 10 ng/mL Recombinant Human beta‑NGF.
Molecular Mass : 120 kDa, 90 kDa and 37 kDa, reducing conditions
N-terminal Sequence Analysis : Met & Ser770
Endotoxin : < 0.1 EU/μg of the protein by the LAL method.
Purity : > 90 % by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Reconstitution : Reconstitute at 250 μg/mL in sterile PBS.
Shipping : The product is shipped with polar packs.
Gene Name SEMA3A semaphorin 3A [ Homo sapiens (human) ]
Official Symbol SEMA3A
Synonyms SEMA3A; semaphorin 3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; semaphorin-3A; collapsin 1; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; semaphorin D; semaphorin III; semaphorin L
Gene ID 10371
mRNA Refseq NM_006080
Protein Refseq NP_006071
MIM 603961
UniProt ID Q14563

Not For Human Consumption!

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All SEMA3A Products

Required fields are marked with *

My Review for All SEMA3A Products

Required fields are marked with *

0

Inquiry Basket

cartIcon