Recombinant Human RSPO2 HEK293T cell lysate

• Cat.No.: RSPO2-42HCL
• Product Overview: Human RSPO2 was derived from Human HEK293T cell line.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a member of the R-spondin family of proteins. These proteins are secreted ligands of leucine-rich repeat containing G protein-coupled receptors that enhance Wnt signaling through the inhibition of ubiquitin E3 ligases. A chromosomal translocation including this locus that results in the formation of a gene fusion has been identified in multiple human cancers. Alternative splicing results in multiple transcript variants.
• Form: The cell lysate is provided in RIPA buffer.
• Molecular Mass: 28.1 kDa
• Usage: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: RSPO2 R-spondin 2 [ Homo sapiens ]
• Official Symbol: RSPO2
• Synonyms: RSPO2; R-spondin 2; R spondin 2 homolog (Xenopus laevis); R-spondin-2; MGC35555; R-spondin 2 homolog; roof plate-specific spondin-2; CRISTIN2; MGC43342
• Gene ID: 340419
• mRNA Refseq: NM_178565
• Protein Refseq: NP_848660
• MIM: 610575
• UniProt ID: Q6UXX9
• Chromosome Location: 8q23.1
• Function: heparin binding