Recombinant Human RPN1 protein, His-tagged

• Cat.No.: RPN1-8053H
• Product Overview: Recombinant Human RPN1 aa. (Ala24~Ser179 (Accession # P04843)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala24~Ser179
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: RPN1 ribophorin I [ Homo sapiens (human) ]
• Official Symbol: RPN1
• Synonyms: OST1; RBPH1; RPN-I; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); ribophorin-1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• Gene ID: 6184
• mRNA Refseq: NM_002950.3
• Protein Refseq: NP_002941.1
• UniProt ID: P04843