Recombinant Human RPN1 protein, His-tagged

• Cat.No.: RPN1-8054H
• Product Overview: Recombinant Human RPN1 aa. (Arg180~Ile307 (Accession # P04843)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg180~Ile307
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: RPN1 ribophorin I [ Homo sapiens (human) ]
• Official Symbol: RPN1
• Synonyms: OST1; RBPH1; RPN-I; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); ribophorin-1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• Gene ID: 6184
• mRNA Refseq: NM_002950.3
• Protein Refseq: NP_002941.1
• UniProt ID: P04843