Recombinant Mouse Rpn1 Protein, MYC/DDK-tagged

• Cat.No.: Rpn1-241M
• Product Overview: Purified recombinant protein of full-length mouse ribophorin I (Rpn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 69 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rpn1 ribophorin I [ Mus musculus (house mouse) ]
• Official Symbol: Rpn1
• Synonyms: Rpn1; ribophorin I; Rpn-1; AU018702; D6Wsu137e; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; RPN-I; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; ribophorin-1; EC 2.4.99.18
• Gene ID: 103963
• mRNA Refseq: NM_133933
• Protein Refseq: NP_598694
• UniProt ID: Q91YQ5