Recombinant Human ROCK2 protein, His & T7-tagged

• Cat.No.: ROCK2-1878H
• Product Overview: Recombinant Human ROCK2 aa. (Leu904~Gly1140 (Accession # O75116)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.1kDa.
• Protein length: Leu904~Gly1140
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ROCK2 Rho associated coiled-coil containing protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ROCK2
• Synonyms: IL17D; interleukin 17D; IL-17D; interleukin-17D
• Gene ID: 9475
• mRNA Refseq: NM_001321643.1
• Protein Refseq: NP_001308572.1
• UniProt ID: O75116