Recombinant Mouse Rock2 Protein, Myc/DDK-tagged

• Cat.No.: Rock2-5568M
• Product Overview: Purified recombinant protein of mouse full-length Rho-associated coiled-coil containing protein kinase 2 (Rock2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 161.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rock2 Rho-associated coiled-coil containing protein kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Rock2
• Synonyms: ROCK2; Rho-associated coiled-coil containing protein kinase 2; rho-associated protein kinase 2; p164 ROCK-2; Rho-associated coiled-coil forming kinage 2; rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II; Rock2m; Rock-II; ROKalpha; mKIAA0619; Rho-kinase; B230113H15Rik
• Gene ID: 19878
• mRNA Refseq: NM_009072
• Protein Refseq: NP_033098
• UniProt ID: P70336