Active Recombinant Human ROCK2 Protein, His-tagged

• Cat.No.: ROCK2-1879H
• Product Overview: Recombinant human ROCK2 (5-554) was expressed using baculovirus in Sf9 insect cells with an N-terminal His tag.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Source: Insect cells
• Species: Human
• Tag: His
• Bio-activity: 605 nmol/min/mg
• Molecular Mass: ~66 kDa
• Purity: >95%
• Stability: One year at -70 centigrade from the date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Shipping: Shipped on dry ice.
• Protein length: 5-554 a.a.

Gene Information

• Gene Name: ROCK2 Rho associated coiled-coil containing protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ROCK2
• Synonyms: ROCK2; Rho associated coiled-coil containing protein kinase 2; ROCK-II; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II; EC 2.7.11.1; rho-associated protein kinase 2
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• UniProt ID: O75116