Recombinant Human ribonuclease, RNase A family, 3, His-tagged

• Cat.No.: RNASE3-370H
• Product Overview: RNASE3, 28-160 aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 28-160 a.a.
• Description: RNASE3 is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. It possesses a wide variety of biological activities. This protein exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Recombinant human RNASE3 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 19.9 kDa (171aa)
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RNASE3 ribonuclease, RNase A family, 3 [ Homo sapiens ]
• Official Symbol: RNASE3
• Synonyms: RNASE3; ribonuclease, RNase A family, 3; RNS3; eosinophil cationic protein; ECP; RNase 3; ribonuclease 3; cytotoxic ribonuclease
• Gene ID: 6037
• mRNA Refseq: NM_002935
• Protein Refseq: NP_002926
• MIM: 131398
• UniProt ID: P12724
• Chromosome Location: 14q24-q31
• Pathway: Asthma, organism-specific biosystem; Asthma, conserved biosystem
• Function: endonuclease activity; hydrolase activity; nucleic acid binding; pancreatic ribonuclease activity; ribonuclease activity