| Species : |
Human |
| Source : |
HEK293 |
| Protein Length : |
67-406 |
| Description : |
This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis. |
| Molecular Mass : |
40 kDa |
| Purity : |
≥85% estimated by SDS-PAGE |
| Unit Definition : |
One unit is defined as the amount of enzyme that will liberate 1.0 nmol of EDANS per minute at 37 centigrade in 50 mM Tris-HCl, pH 8.0, containing 100 nM NaCl, and 10 μM of the fluorescent peptide Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-ThrLys(dabcyl)-Arg. Renin is diluted with 50 mM Tris-HCl, pH 8.0, containing 100 nM NaCl before assaying in a final assay volume of 205 μL with a fluorescence microplate reader using an excitation wavelength of 340 nm and emission wavelength of 485 nm. |
| Stability : |
≥ 1 year |
| Storage : |
Store it at -80 centigrade |
| Storage Buffer : |
50 mM Tris, pH 8.0, with 150 mM NaCl and 5% glycerol |
