Recombinant Human Prorenin, His-tagged

• Cat.No.: REN -16H
• Product Overview: Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Fully activatable to renin by catalytic amounts of trypsin.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Description: Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site . Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects.
• Form: Frozen liquid
• Molecular Mass: 43,725
• Purity: >95% by SDS-PAGE analysis
• Usage: For in vitro laboratory use only
• Notes: Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
• Storage: -70°C
• Concentration: 1.0 mg/ml
• Storage Buffer: 50mM Tris; pH 8.0
• Shipping: Dry ice

Gene Information

• Gene Name: REN renin [ Homo sapiens ]
• Official Symbol: REN
• Synonyms: REN; renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; HNFJ2; FLJ10761
• Gene ID: 5972
• mRNA Refseq: NM_000537
• Protein Refseq: NP_000528
• MIM: 179820
• UniProt ID: P00797
• Chromosome Location: 1q32
• Pathway: ACE Inhibitor Pathway, organism-specific biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem
• Function: aspartic-type endopeptidase activity; insulin-like growth factor receptor binding; peptidase activity; receptor binding