Recombinant Human RARA 293 Cell Lysate

• Cat.No.: RARA-2515HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for retinoic acid receptor, alpha (RARA), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RARA retinoic acid receptor, alpha [ Homo sapiens ]
• Official Symbol: RARA
• Synonyms: RARA; retinoic acid receptor, alpha; retinoic acid receptor alpha; NR1B1; RAR; RAR-alpha; retinoic acid receptor, alpha polypeptide; nuclear receptor subfamily 1 group B member 1; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form
• Gene ID: 5914
• mRNA Refseq: NM_001024809
• Protein Refseq: NP_001019980
• MIM: 180240
• UniProt ID: P10276
• Chromosome Location: 17q21.1
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adipogenesis, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; IL-3 Signaling Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem
• Function: chromatin DNA binding; enzyme binding; metal ion binding; protein binding; protein domain specific binding; protein heterodimerization activity; protein kinase A binding; protein kinase B binding; receptor activity; receptor binding; retinoic acid binding; retinoic acid receptor activity; retinoic acid-responsive element binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; transcription coactivator activity; transcription coactivator activity; transcription corepressor activity; transcription factor binding; zinc ion binding