Recombinant Human Retinoic Acid Receptor, Alpha, His-tagged

• Cat.No.: RARA-190H
• Product Overview: Recombinant Human Retinoic Acid Receptor Alpha (His-tagged) produced inE.Coliis a single, non-glycosylated polypeptide chain. The protein contains 127 amino acids and has a molecular mass of 14 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Retinoic acid receptor alpha (RAR) belongs to the large family of ligand responsive gene regulatory proteins that includes receptors for steroid and thyroid hormones. These proteins contain two highly conserved domains that are involved in determining their DNA and ligand-binding activities. There are three isotypes of RAR proteins: alpha, beta, and gamma. The RAR proteins are encoded by distinct genetic loci and possess distinct transcriptional properties. Typically, RAR-alpha represses target gene transcription in the absence of hormone, whereas RAR-beta and gamma fail to repress under these conditions. RARA is a receptor for retinoic acid that has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. RARA controls cell function by directly regulating gene expression. Chromosomal aberrations involving RARA cause acute promyelocytic leukemia (APL).
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSEEIVPSPP SPPPLPRIYK PCFVCQDKSS GYHYGVSACE GCKGFFRRSI QKNMVYTCHR DKNCIINKVT RNRCQYCRLQ KCFEVGMSKESVRNDRNKKK KEVPKPE.
• Physical Appearance: Sterile Filtered colorless solution.
• Formulation: The protein solution cotains 20mM Tris pH-7.5, 0.1M NaCl & 5mMβ-ME.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Stability: RARA although stable 14°Cfor 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: RARA retinoic acid receptor, alpha [ Homo sapiens ]
• Synonyms: RARA; NR1B1; RAR; RAR-alpha; Retinoic acid receptor, alpha; Retinoic acid receptor alpha; OTTHUMP00000164454; OTTHUMP00000164456; Nuclear receptor subfamily 1 group B member 1; Retinoic acid receptor, alpha polypeptide; retinoic acid receptor, alpha; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; Retinoic acid receptor alpha (RAR-alpha); NR1B1 (nuclear receptor subfamily 1, group B, member 1)
• Gene ID: 5914
• mRNA Refseq: NM_000964
• Protein Refseq: NP_000955
• MIM: 180240
• UniProt ID: P10276
• Chromosome Location: 17q21
• Pathway: Acute myeloid leukemia; Pathways in cancer
• Function: metal ion binding; protein binding; retinoic acid binding; retinoic acid receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; transcription coactivator activity; transcription factor activity; zinc ion binding