Recombinant Human QPCT protein, His-tagged

• Cat.No.: QPCT-36H
• Product Overview: Recombinant human QPCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 29-361 a.a.
• Description: QPCT, also known as glutaminyl-peptide cyclotransferase, is responsible for the biosynthesis of pyroglutamyl peptides. This protein has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. It catalyzes N-terminal pyroglutamate formation. In vitro, it catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 38.7kDa (339aa)28-40kDa (SDS-PAGE under reducing conditions)
• AA Sequence: VSPSASAWPE EKNYHQPAIL NSSALRQIAE GTSISEMWQN DLQPLLIERY PGSPGSYAAR QHIMQRIQRL QADWVLEIDT FLSQTPYGYRSFSNIISTLN PTAKRHLVLA CHYDSKYFSH WNNRVFVGAT DSAVPCAMML ELARALDKKL LSLKTVSDSK PDLSLQLIFF DGEEAFLHWSPQDSLYGSRH LAAKMASTPH PPGARGTSQL HGMDLLVLLD LIGAPNPTFP NFFPNSARWF ERLQAIEHEL HELGLLKDHS LEGRYFQNYSYGGVIQDDHI PFLRRGVPVL HLIPSPFPEV WHTMDDNEEN LDESTIDNLN KILQVFVLEY LHLHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS – PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: QPCT glutaminyl-peptide cyclotransferase [ Homo sapiens ]
• Official Symbol: QPCT
• Synonyms: QPCT; glutaminyl-peptide cyclotransferase; GCT; glutaminyl cyclase; QC; EC; glutamyl cyclase; glutaminyl-tRNA cyclotransferase; sQC
• Gene ID: 25797
• mRNA Refseq: NM_012413
• Protein Refseq: NP_036545
• MIM: 607065
• UniProt ID: Q16769
• Chromosome Location: 2p22
• Function: glutaminyl-peptide cyclotransferase activity; metal ion binding; peptidase activity; transferase activity, transferring acyl groups; zinc ion binding