Recombinant Human PYCR2 293 Cell Lysate

• Cat.No.: PYCR2-2647HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for pyrroline-5-carboxylate reductase family, member 2 (PYCR2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PYCR2 pyrroline-5-carboxylate reductase family, member 2 [ Homo sapiens ]
• Official Symbol: PYCR2
• Synonyms: PYCR2; pyrroline-5-carboxylate reductase family, member 2; pyrroline-5-carboxylate reductase 2; P5CR2; P5CR 2; P5C reductase 2; pyrroline 5-carboxylate reductase isoform; FLJ54750
• Gene ID: 29920
• mRNA Refseq: NM_013328
• Protein Refseq: NP_037460
• UniProt ID: Q96C36
• Chromosome Location: 1q42.13
• Pathway: Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Proline biosynthesis, glutamate =>proline, organism-specific biosystem; Proline biosynthesis, glutamate => proline, conserved biosystem
• Function: nucleotide binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; pyrroline-5-carboxylate reductase activity