Recombinant Human Prolactin Receptor / PRLR protein, His-tagged

• Cat.No.: PRLR-2060H
• Product Overview: Recombinant Human PRLR (Gln25-Asp234) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: As a transmembrane receptor, the prolactin receptor (PRL-R) interacts with prolactin. In addition, the PRL-R also binds and is activated by growth hormone (GH) and human placental lactogen (hPL). Preclinical investigations, epidemiological studies and analyses of tissue specimens from patients strongly support the contribution of prolactin receptor (PRLR) signaling to breast and prostate tumorigenesis and cancer progression. Moreover, The PRLR has been found to be essential for lobuloalveolar maturation of the mammary glands during pregnancy, as evidenced by the fact that PRLR knockout mice show severely impaired development of lobuloalveolar structures.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Gln25
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 , 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 26.3 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 25-234 a.a.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PRLR
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein; hPRLrI
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471