Recombinant Human PRDM1 protein, His-tagged

• Cat.No.: PRDM1-1758H
• Product Overview: Recombinant Human PRDM1 aa. (Leu29~Asp149) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Leu29~Asp149
• Description: This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 4.9

Gene Information

• Gene Name: PRDM1 PR/SET domain 1 [ Homo sapiens (human) ]
• Official Symbol: PRDM1
• Synonyms: PRDM1; PR/SET domain 1; BLIMP1; PRDI-BF1; PR domain zinc finger protein 1; B-lymphocyte-induced maturation protein 1; BLIMP-1; PR domain 1; PR domain containing 1, with ZNF domain; PRDI-binding factor-1; beta-interferon gene positive-regulatory domain I binding factor
• Gene ID: 639
• mRNA Refseq: NM_001198.3
• Protein Refseq: NP_001189.2
• UniProt ID: O75626