Recombinant Human PPP2R4 293 Cell Lysate

• Cat.No.: PPP2R4-2919HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for protein phosphatase 2A activator, regulatory subunit 4 (PPP2R4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PPP2R4 protein phosphatase 2A activator, regulatory subunit 4 [ Homo sapiens ]
• Official Symbol: PPP2R4
• Synonyms: PPP2R4; protein phosphatase 2A activator, regulatory subunit 4; protein phosphatase 2A, regulatory subunit B (PR 53); serine/threonine-protein phosphatase 2A activator; phosphotyrosyl phosphatase activator; PP2A phosphatase activator; PR53; PTPA; PP2A subunit B isoform PR53; serine/threonine-protein phosphatase 2A regulatory subunit B; PP2A; MGC2184
• Gene ID: 5524
• mRNA Refseq: NM_001193397
• Protein Refseq: NP_001180326
• MIM: 600756
• UniProt ID: Q15257
• Chromosome Location: 9q34
• Pathway: Glycogen Metabolism, organism-specific biosystem; IL-6 Signaling Pathway, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem; Wnt Signaling Pathway and Pluripotency, organism-specific biosystem; p53 pathway, organism-specific biosystem
• Function: ATP binding; contributes_to ATPase activity; isomerase activity; nucleotide binding; peptidyl-prolyl cis-trans isomerase activity; phosphatase activator activity; protein heterodimerization activity; protein homodimerization activity; protein phosphatase 2A binding; protein phosphatase type 2A regulator activity; protein phosphatase type 2A regulator activity; protein tyrosine phosphatase activator activity; receptor binding