Recombinant Human PPM1F, His-tagged

• Cat.No.: PPM1F-2281H
• Product Overview: Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
• Molecular Mass: 52kDa
• Protein length: 477aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLGSRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAKRERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGEGDPQAEGRRQ DLPSSLPEPE TQAPPRS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: PPM1F protein phosphatase, Mg2+/Mn2+ dependent, 1F [ Homo sapiens ]
• Official Symbol: PPM1F
• Synonyms: PPM1F; protein phosphatase, Mg2+/Mn2+ dependent, 1F; protein phosphatase 1F (PP2C domain containing); protein phosphatase 1F; Ca(2+)/calmodulin dependent protein kinase phosphatase; CAMKP; CaMKPase; FEM 2; KIAA0015; partner of PIX 2; POPX2; PP2C phosphatase; protein fem-2 homolog; CaM-kinase phosphatase; Ca(2+)/calmodulin-dependent protein kinase phosphatase; FEM-2; hFEM-2
• Gene ID: 9647
• mRNA Refseq: NM_014634
• Protein Refseq: NP_055449
• UniProt ID: P49593
• Chromosome Location: 22q11.22
• Function: calmodulin-dependent protein phosphatase activity; hydrolase activity; metal ion binding; phosphatase activity; protein binding; protein serine/threonine phosphatase activity; protein serine/threonine phosphatase activity