Recombinant Human POLR2J3 polymerase (RNA) II (DNA directed) polypeptide J3, His-tagged

• Cat.No.: POLR2J3-7175H
• Product Overview: Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 15.5 kDa.

Specification

• Description: DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol,1mM DTT.
• Molecular Mass: 15.5 kDa (138aa) confirmed by MALDI-TOF
• Protein length: 1-115aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLR2J3 polymerase (RNA) II (DNA directed) polypeptide J3 [ Homo sapiens ]
• Official Symbol: POLR2J3
• Synonyms: POLR2J3; polymerase (RNA) II (DNA directed) polypeptide J3; POLR2J2; RPB11b1; RPB11b2; DNA-directed RNA polymerase II subunit RPB11-b2; RNA polymerase II subunit B11-b2; DNA-directed RNA polymerase II subunit 11; DNA-directed RNA polymerase II subunit J3
• Gene ID: 548644
• mRNA Refseq: NM_001097615
• Protein Refseq: NP_001091084
• UniProt ID: Q9GZM3
• Chromosome Location: 7q22.1
• Pathway: Epstein-Barr virus infection, organism-specific biosystem; Huntington"s disease, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem; RNA polymerase, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity