Recombinant Human POLK, His-tagged

• Cat.No.: POLK-838H
• Product Overview: Recombinant Human POLK, fused with His tag C-Terminus, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1 to 560
• Description: External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass.
• Form: Liquid
• Molecular Mass: 64 kDa
• AA Sequence: MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAKELLKTEIDADF PHPLRLRLMGVRISSFPNEEDRKHQQRSIIGFLQAGNQALSATECTLEKT DKDKFVKPLE
• Purity: >90% by SDS-PAGE.
• Applications: ELISA; SDS-PAGE; Western blot; Functional Studies
• Storage: Store at -20°C or -80°C.
• Concentration: 3.2 mg/ml

Gene Information

• Gene Name: POLK?polymerase (DNA directed) kappa [?Homo sapiens?(human) ]
• Official Symbol: POLK
• Synonyms: POLK; DINP; POLQ; DINB1; polymerase (DNA directed) kappa; DNA polymerase kappa; NP_057302.1; EC 2.7.7.7
• Gene ID: 51426
• mRNA Refseq: NM_016218
• Protein Refseq: NP_057302
• MIM: 605650
• UniProt ID: Q9UBT6
• Chromosome Location: 5q13
• Pathway: Fanconi anemia pathway
• Function: DNA-directed DNA polymerase activity; damaged DNA binding; metal ion binding