Recombinant Human POLB cell lysate

• Cat.No.: POLB-1388HCL

Specification

• Species: Human
• Tag: Non
• Description: In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. Also see POLA (MIM 312040).
• Size: 100 ul
• Storage Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
• Applications: Western Blot

Gene Information

• Gene Name: POLB polymerase (DNA directed), beta [ Homo sapiens ]
• Official Symbol: POLB
• Synonyms: POLB; polymerase (DNA directed), beta; DNA polymerase beta; DNA pol beta; DNA polymerase beta subunit; MGC125976
• Gene ID: 5423
• mRNA Refseq: NM_002690
• Protein Refseq: NP_002681
• MIM: 174760
• UniProt ID: P06746
• Chromosome Location: 8p12-p11
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem; DNA Repair, organism-specific biosystem
• Function: DNA-directed DNA polymerase activity; damaged DNA binding; enzyme binding; lyase activity; metal ion binding; microtubule binding; nucleotidyltransferase activity; protein binding; transferase activity

Reviews

09/07/2023

Efficacy: The enzyme is still active and I am currently using it in some DNA repair assays. It works very well for single nucleotide insertions in gapped DNA which is mostly what I have been using it for recently. Quality: Very happy with the quality and longevity. I think the enzyme is ~1yr old with frequent usage and it is still working fine. Overall Experience: Very happy. When we need more polB/hUNG, and potentially others, we will purchase from you again.