Recombinant Human POLB protein

• Cat.No.: POLB-621H
• Product Overview: Recombinant Human POLB protein was a repair enzyme used to fill gaps and nicks in double-stranded DNA.

Specification

• Species: Human
• Tag: Non
• Form: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA. 200 mM NaCl, 1 mM dithiothreitol, 50% (v/v) glycerol
• Unit Definition: One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37 centigrade under the stated assay conditions.
• Applications: 1. Repair polymerase able to synthesize DNA beyond the end of a gap or nick with simultaneous displacement of the non-replicated strand.2. Fills gaps and nicks.
• Notes: All preparations are assayed for contaminating endonuclease and exonuclease activities.
• Storage: Store at -80 centigrade Long Term;Store at -20 centigrade.

Gene Information

• Gene Name: POLB polymerase (DNA directed), beta [ Homo sapiens ]
• Official Symbol: POLB
• Synonyms: POLB; polymerase (DNA directed), beta; DNA polymerase beta; DNA pol beta; DNA polymerase beta subunit; MGC125976
• Gene ID: 5423
• mRNA Refseq: NM_002690
• Protein Refseq: NP_002681
• MIM: 174760
• UniProt ID: P06746
• Chromosome Location: 8p12-p11
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem; DNA Repair, organism-specific biosystem
• Function: DNA-directed DNA polymerase activity; damaged DNA binding; enzyme binding; lyase activity; metal ion binding; microtubule binding; nucleotidyltransferase activity; protein binding; transferase activity

Reviews

09/07/2023

Efficacy: The enzyme is still active and I am currently using it in some DNA repair assays. It works very well for single nucleotide insertions in gapped DNA which is mostly what I have been using it for recently. Quality: Very happy with the quality and longevity. I think the enzyme is ~1yr old with frequent usage and it is still working fine. Overall Experience: Very happy. When we need more polB/hUNG, and potentially others, we will purchase from you again.