Recombinant Human PLD2, GST-tagged

• Cat.No.: PLD2-679H
• Product Overview: Recombinant Human PLD2(1 a.a. - 933 a.a.), fused with GST-tag at N-terminal, was expressed in wheat germ.

Specification

• Species: Human
• Source: Wheat Germ
• Tag: GST
• Description: The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 132.3 kDa
• AA Sequence: MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERY TSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRA GPEGSTRHAASKQKYLENYLNCLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCG RDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQAR WWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRP AHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQ VVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRP FEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPIYPYLLPKSTSTANQLPFTLPGGQ CTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCY RVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVS ELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILG ANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQ GHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT
• Applications: ELISA; WB-Re; AP; Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: PLD2 phospholipase D2 [ Homo sapiens (human) ]
• Official Symbol: PLD2
• Synonyms: PLD2; phospholipase D2; PLD1C; hPLD2; choline phosphatase 2; phosphatidylcholine-hydrolyzing phospholipase D2; NP_001230037.1; EC 3.1.4.4; NP_002654.3
• Gene ID: 5338
• mRNA Refseq: NM_002663
• Protein Refseq: NP_002654
• MIM: 602384
• UniProt ID: O14939
• Chromosome Location: 17p13.1
• Pathway: Alpha-synuclein signaling; Arf6 downstream pathway; Arf6 trafficking events; CDC42 signaling events; EGFR1 Signaling Pathway
• Function: N-acylphosphatidylethanolamine-specific phospholipase D activity; phosphatidylinositol binding; phospholipase D activity; protein binding