Recombinant Human PLA2G10 293 Cell Lysate

• Cat.No.: PLA2G10-3145HCL

Specification

• Description: Antigen standard for phospholipase A2, group X (PLA2G10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: PLA2G10 phospholipase A2, group X [ Homo sapiens ]
• Official Symbol: PLA2G10
• Synonyms: PLA2G10; phospholipase A2, group X; group 10 secretory phospholipase A2; GXPLA2; sPLA2-X; GX sPLA2; group X secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 10; SPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367
• Gene ID: 8399
• mRNA Refseq: NM_003561
• Protein Refseq: NP_003552
• MIM: 603603
• UniProt ID: O15496
• Chromosome Location: 16p13.1-p12
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem
• Function: calcium ion binding; hydrolase activity; phospholipase A2 activity; phospholipase activity