Recombinant Human Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, GST-tagged

• Cat.No.: PIP5K1A-1082H
• Product Overview: Recombinant full-length human PIP5K1A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4, 5-bisphosphate. PIP5K1A is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through "frizzled" and "dishevelled", the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 86 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF,0 25% glycerol.
• Specific Activity: 1310 nmol/min/mg
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: 3-phosphoinositide biosynthesis; Arf1 pathway; Arf6 downstream pathway; B Cell Receptor Signaling Pathway; D-myo-inositol (1,4,5)-trisphosphate biosynthesis; E-cadherin signaling in keratinocytes; Endocytosis; Fc gamma R-mediated phagocytosis; Inositol phosphate metabolism; Metabolic pathways; Osteopontin-mediated events; Phosphatidylinositol signaling system; RAC1 signaling pathway; Regulation of actin cytoskeleton; RhoA signaling pathway

Gene Information

• Gene Name: PIP5K1A phosphatidylinositol-4-phosphate 5-kinase, type I, alpha [ Homo sapiens ]
• Official Symbol: PIP5K1A
• Synonyms: PIP5K1A; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; PIP5KIalpha; PIP5K1-alpha; OTTHUMP00000014289; OTTHUMP00000014290; OTTHUMP00000014291; OTTHUMP00000196331; OTTHUMP00000235065; ptdIns(4)P-5-kinase 1 alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha
• Gene ID: 8394
• mRNA Refseq: NM_001135636
• Protein Refseq: NP_001129108
• MIM: 603275
• UniProt ID: Q99755
• Chromosome Location: 1q21.3
• Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding; kinase activity; kinase binding; nucleotide binding; protein binding; transferase activity