Active Recombinant Human PIP5K1A, GST-tagged

• Cat.No.: PIP5K1A-1460H
• Product Overview: Recombinant full-length human PIP5K1A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through frizzled and dishevelled, the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 1310nmol/min/mg
• Molecular Mass: ~86 kDa
• Protein length: Full length
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PIP5K1A phosphatidylinositol-4-phosphate 5-kinase, type I, alpha [ Homo sapiens ]
• Official Symbol: PIP5K1A
• Synonyms: PIP5K1A; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; PIP5KIalpha; PIP5K1-alpha; ptdIns(4)P-5-kinase 1 alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha
• Gene ID: 8394
• mRNA Refseq: NM_001135636
• Protein Refseq: NP_001129108
• MIM: 603275
• UniProt ID: Q99755
• Chromosome Location: 1q21.3
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Arf1 pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem
• Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding; kinase binding; nucleotide binding; protein binding; transferase activity