Recombinant Human PDZ and LIM domain 3 Protein, His&Flag&StrepII tagged

• Cat.No.: PDLIM3-071H
• Product Overview: Recombinant human PDLIM3/ALP protein (Q53GG5) (Asp 111-Ser 231) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: N-Twin strep and C-His&Flag
• ProteinLength: 111-231aa
• Description: The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy. May play a role in the organization of actin filament arrays within muscle cells.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 19.4 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: PDLIM3 PDZ and LIM domain 3 [ Homo sapiens (human) ]
• Official Symbol: PDLIM3
• Synonyms: PDLIM3; PDZ and LIM domain 3; PDZ and LIM domain protein 3; ALP; enigma homolog; alpha-actinin-2-associated LIM protein; FLJ26096; DKFZp686L0362
• Gene ID: 27295
• mRNA Refseq: NM_001114107
• Protein Refseq: NP_001107579
• MIM: 605889
• UniProt ID: Q53GG5