Recombinant Human PDGFA

• Cat.No.: PDGFA-121H
• Product Overview: Recombinant Human Platelet-Derived Growth Factor AA is a homodimeric, non-glycosylated produced inE.Coli, polypeptide chain containing 2 x 125 amino acids and having a total molecular mass of 28511 Dalton.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC; (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized with no additives.
• Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg.
• Stability: Lyophilized Platelet-Derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PDGFA platelet-derived growth factor alpha polypeptide [ Homo sapiens ]
• Synonyms: platelet-derived growth factor alpha polypeptide; PDGF1; PDGF-A; PDGFA; platelet-derived growth factor alpha; PDGF A-chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein; PDGF1; PDGF subunit A; Platelet-derived growth factor A chain; platelet-derived growth factor alpha polypeptide
• Gene ID: 5154
• mRNA Refseq: NM_002607
• Protein Refseq: NP_002598
• MIM: 173430
• UniProt ID: P04085
• Chromosome Location: 7p22
• Pathway: Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis
• Function: cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; contributes_to platelet-derived growth factor receptor binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity