Active Recombinant Human PDGFA Protein

• Cat.No.: PDGFA-094H
• Product Overview: The mature recombinant human PDGF-AB disulfide-linked dimer containing one A chain and one B chain (234aa) without tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Bio-activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/mL, corresponding to a specific activity of ≥ 1 x 106 units/mg.
• Molecular Mass: 12.3 kDa
• Endotoxin: < 0.1 EU/μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: At -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: PBS

Gene Information

• Gene Name: PDGFA platelet derived growth factor subunit A [ Homo sapiens (human) ]
• Official Symbol: PDGFA
• Synonyms: PDGFA; platelet derived growth factor subunit A; PDGF1; PDGF-A; platelet-derived growth factor subunit A; Names; PDGF A-chain; PDGF subunit A; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha polypeptide
• Gene ID: 5154
• mRNA Refseq: NM_002607
• Protein Refseq: NP_002598
• MIM: 173430
• UniProt ID: P04085