Recombinant Human PDE10A 293 Cell Lysate

• Cat.No.: PDE10A-3355HCL

Specification

• Description: Antigen standard for phosphodiesterase 10A (PDE10A), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: PDE10A phosphodiesterase 10A [ Homo sapiens ]
• Official Symbol: PDE10A
• Synonyms: PDE10A; phosphodiesterase 10A; cAMP and cAMP-inhibited cGMP 3,5-cyclic phosphodiesterase 10A; phosphodiesterase 10A1 (PDE10A1); dJ416F21.1 (phosphodiesterase 10A); HSPDE10A; FLJ11894; FLJ25677
• Gene ID: 10846
• mRNA Refseq: NM_001130690
• Protein Refseq: NP_001124162
• MIM: 610652
• UniProt ID: Q9Y233
• Chromosome Location: 6q26
• Pathway: G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Hemostasis, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Platelet homeostasis, organism-specific biosystem
• Function: 3,5-cyclic-GMP phosphodiesterase activity; 3,5-cyclic-nucleotide phosphodiesterase activity; cAMP binding; cAMP binding; cGMP binding; cGMP-stimulated cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding; nucleotide binding; phosphoric diester hydrolase activity