Recombinant Human PCOLCE protein, His & S-tagged

• Cat.No.: PCOLCE-6802H
• Product Overview: Recombinant Human PCOLCE aa. (Ala315~Cys437 (Accession # Q15113)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Description: Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.
• Source: E. coli
• Species: Human
• Tag: His&S
• Form: Freeze-dried powder
• Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala315~Cys437
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PCOLCE procollagen C-endopeptidase enhancer [ Homo sapiens (human) ]
• Official Symbol: PCOLCE
• Synonyms: PCPE; PCPE1; PCPE-1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer 1
• Gene ID: 5118
• mRNA Refseq: NM_002593.3
• Protein Refseq: NP_002584.2
• UniProt ID: Q15113