Recombinant Human paired box 8, His-tagged

• Cat.No.: PAX8-675H
• Product Overview: PAX8, 1-287aa Human, His tag, E.coli

Specification

• Description: Paired box protein Pax-8 isoform PAX8E, also known as PAX8, is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. PAX8complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2). Recombinant human PAX8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In Phosphate buffer saline (pH 7.4) containing 20% glycerol, 1mM DTT
• Molecular Mass: 33.4 kDa (310aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPHNSIR SGHGGLNQLG GAFVNGRPLP EVVRQRIVDL AHQGVRPCDI SRQLRVSHGC VSKILGRYYE TGSIRPGVIG GSKPKVATPK VVEKIGDYKR QNPTMFAWEI RDRLLAEGVC DNDTVPSVSS INRIIRTKVQ QPFNLPMDSC VATKSLSPGH TLIPSSAVTP PESPQSDSLG STYSINGLLG IAQPGSDKRK MDDSDQDSCR LSIDSQSSSS GPRKHLRTDA FSQHHLEPLE CPFERQHYPE AYASPSHTKG EQEVNTLAMP MATPPTPPTA RPGASPTPAC
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Protein length: 1-287 a.a.

Gene Information

• Gene Name: PAX8 paired box 8 [ Homo sapiens ]
• Official Symbol: PAX8
• Synonyms: PAX8; paired box 8; paired box gene 8; paired box protein Pax-8; paired domain gene 8
• Gene ID: 7849
• mRNA Refseq: NM_003466
• Protein Refseq: NP_003457
• MIM: 167415
• UniProt ID: Q06710
• Chromosome Location: 2q13
• Pathway: Id Signaling Pathway, organism-specific biosystem; Pathways in cancer, organism-specific biosystem; Thyroid cancer, organism-specific biosystem; Thyroid cancer, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: DNA binding; DNA binding; RNA polymerase II core promoter sequence-specific DNA binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; thyroid-stimulating hormone receptor activity; transcript