Recombinant Human NUDT3, His-tagged

• Cat.No.: NUDT3-3476H
• Product Overview: Recombinant human NUDT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NUDT3, also known as DIPP, DIPP1 (diphosphoinositol polyphosphate phosphohydrolase 1), is a 172 amino acid cytoplasmic protein belonging to the nudix hydrolase family and DIPP subfamily. NUDT3 acts as a negative regulator of the ERK 1/2 pathway and hydrolyzes 5-phosphoribose 1-diphosphate. NUDT3 exists as a monomer and binds magnesium as a cofactor. Also NUDT3 is widely expressed but found at highest levels in liver, pancreas, brain and heart.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
• Molecular Weight: 21.6 kDa (192aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MMKLKSNQTR TYDGDGYKKR AACLCFRSES EEEVLLVSSS RHPDRWIVPG GGMEPEEEPS VAAVREVCEE AGVKGTLGRL VGIFENQERK HRTYVYVLIV TEVLEDWEDS VNIGRKREWF KIEDAIKVLQ YHKPVQASYF ETLRQGYSAN NGTPVVATTY SVSAQSSMSG IR
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NUDT3 nudix (nucleoside diphosphate linked moiety X)-type motif 3 [ Homo sapiens ]
• Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 3; DIPP; DIPP1; NUDT3; diphosphoinositol polyphosphate phosphohydrolase 1; DIPP-1; nudix motif 3; nucleoside diphosphate-linked moiety X motif 3; diadenosine 5",5""-P1,P6-hexaphosphate hydrolase 1; NP_006694.1; EC 3.6.1.52
• Gene ID: 11165
• mRNA Refseq: NM_006703
• Protein Refseq: NP_006694
• MIM: 609228
• UniProt ID: O95989
• Chromosome Location: 6p21.2
• Function: diphosphoinositol-polyphosphate diphosphatase activity; hydrolase activity; magnesium ion binding