| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His&mFc |
| Protein Length : |
His27-Arg102 |
| Description : |
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The presence of myocardial injury is a significant predictor of mortality in hospitalized coronavirus disease 2019 (COVID-19) patients, and there is evidence of increased levels of natriuretic peptide B in hospitalized non-survivor COVID-19 patients. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. |
| Form : |
Lyophilized powder |
| Molecular Mass : |
Recombinant human NT-proBNP has a calculated molecular mass of 35 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 40-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Purity : |
> 90%, SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Stability : |
Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles. |
| Storage : |
At -20 centigrade. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Shipping : |
Ice packs |
| Reconstitution : |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5-1.0 mg/mL with sterile deionized water or 1×PBS. |
