Recombinant Human NSMAF protein, His & GST-tagged

• Cat.No.: NSMAF-1124H
• Product Overview: Recombinant Human NSMAF aa. (Arg176~Arg575 (Accession # Q92636) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Arg176~Arg575
• Description: This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 76.5kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NSMAF neutral sphingomyelinase activation associated factor [ Homo sapiens (human) ]
• Official Symbol: NSMAF
• Synonyms: NSMAF; neutral sphingomyelinase activation associated factor; FAN; GRAMD5; protein FAN; factor associated with N-SMase activation; factor associated with neutral sphingomyelinase activation; neutral sphingomyelinase (N-SMase) activation associated factor
• Gene ID: 8439
• mRNA Refseq: NM_001144772.1
• Protein Refseq: NP_001138244.1
• UniProt ID: Q92636