Recombinant Mouse Nsmaf Protein, Myc/DDK-tagged

• Cat.No.: Nsmaf-4510M
• Product Overview: Purified recombinant protein of mouse full-length neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.
• Molecular Mass: 104.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nsmaf neutral sphingomyelinase (N-SMase) activation associated factor [ Mus musculus (house mouse) ]
• Official Symbol: Nsmaf
• Synonyms: NSMAF; neutral sphingomyelinase (N-SMase) activation associated factor; protein FAN; factor associated with N-SMase activation; factor associated with neutral sphingomyelinase activation; Fan; AA959567; C630007J05
• Gene ID: 18201
• mRNA Refseq: NM_010945
• Protein Refseq: NP_035075
• UniProt ID: O35242