Recombinant Human NPHS2 protein, His-tagged

• Cat.No.: NPHS2-718H
• Product Overview: Recombinant Human NPHS2(Val125-Leu383) fused with His tag at C-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 125-383 a.a.
• Description: Nephrosis 2 Idiopathic Steroid-Resistant (NPHS2) is a protein which has a role in the regulation of glomerular permeability, serving as a linker between the plasma membrane and the cytoskeleton. NPHS2 gene mutations cause steroid-resistant nephrotic syndrome.
• Form: NPHS2 was filtered (0.4µm) and lyophilized in 30mM acetate buffer and 1mM dithiothreitol, pH 4.
• Molecular Mass: 30.2kDa
• AA Sequence: MKHHHHHHASVKVVQEYERV IIFRLGHLLP GRAKGPGLFF FLPCLDTYHK VDLRLQTLEI PFHEIVTKDM FIMEIDAICY YRMENASLLL SSLAHVSKAV QFLVQTTMKR LLAHRSLTEI LLERKSIAQD AKVALDSVTC IWGIKVERIE IKDVRLPAGL QHSLAVEAEA QRQAKVRMIA AEAEKAASES LRMAAEILSG TPAAVQLRYL HTLQSLSTEK PSTVVLPLPF DLLNCLSSPS NRTQGSLPFP SPSKPVEPLN PKKKDSPML.
• Purity: Purity as determined by densitometric image analysis is greater than 95%.
• Storage: Store lyophilized protein at -20 centigrade. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 centigrade for a limited period of time; it does not show any change after two weeks at 4 centigrade.
• Reconstitution: It is recommended to add 0.1M acetate buffer pH 4.0 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. NPHS2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Gene Information

• Gene Name: NPHS2 NPHS2, podocin [ Homo sapiens ]
• Official Symbol: NPHS2
• Synonyms: PDCN; SRN1; nephrosis 2, idiopathic, steroid-resistant (podocin)
• Gene ID: 7827
• mRNA Refseq: NM_014625
• Protein Refseq: NP_055440
• MIM: 604766
• UniProt ID: Q9NP85
• Chromosome Location: 1q25.2
• Pathway: Nephrin/Neph1 signaling in the kidney podocyte, organism-specific biosystem
• Function: protein binding