Active Recombinant Human NPHS2 protein, His-tagged

• Cat.No.: NPHS2-251H
• Product Overview: Recombinant Human NPHS2(Lys259-Leu383) fused with an N-terminal Met and a C-terminal 6-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 259-383 a.a.
• Description: Podocin, encoded by the NPHS2 gene, is an approximately 50 kDa membrane protein that plays an important role in podocyte function in the kidney. Loss of Podocin function results in albuminuria, hypercholesterolemia, hypertension, and renal failure. Human Podocin consists of a 102 amino acid (aa) cytoplasmic domain, a 21 aa intramembrane segment, and a second 262 aa cytoplasmic domain. Alternative splicing generates a short isoform with a 68 aa deletion in the second cytoplasmic domain. Within aa 259-383 (the region common to both isoforms), human Podocin shares 90% aa sequence identity with mouse and rat Podocin. Podicin localizes to areas of cell-cell contact between podocytes in the renal glomerulus. It associates into oligomers and forms complexes with Nephrin, CAR, ZO-1, and the cation ion channel TRPC6. It contributes to podocyte function by regulating the activation of TRPC6 and Nephrin mediated signaling. Multiple polymorphisms in NPHS2 are associated with steroid-resistant nephrotic syndrome.
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. When recombinant human Nephrin is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Podocin/NPHS2 binds with a typical ED50 of 0.8-4 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 15 kDa
  SDS-PAGE: 13 kDa, rducing conditions
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.

Gene Information

• Gene Name: Nphs2 nephrosis 2 homolog, podocin (human) [ Mus musculus ]
• Official Symbol: NPHS2
• Synonyms: NPHS2; nephrosis 2 homolog, podocin (human); podocin; PDCN; SRN1; AI790225
• Gene ID: 170484
• mRNA Refseq: NM_130456
• Protein Refseq: NP_569723
• UniProt ID: Q91X05