Recombinant Human Neurotrophin 3

• Cat.No.: NTF3-3621H
• Product Overview: Recombinant Human proNT-3 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27.4 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The precursor form of neurotrophin-3 (proNT-3) functions via p75NTR and sortilin and induces neuronal apoptosis. On the contrary, mature NT-3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. As axon retrograde transport is crucial for neurotrophin signaling, proNT-3 also plays an important role in the mechanism of retrograde signaling.
• Biological Activity: As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F10 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml.
• Purity: Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Asn-Met-Asp-Gln.
• Endotoxin: Less than 0.1ng/μg (1.0EU/μg) of rHuproNT-3 as determined by LAL test.
• Form: The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 8.0, 300mM NaCl buffer.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuproNT-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rHuproNT-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuproNT-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: NTF3 neurotrophin 3 [ Homo sapiens ]
• Official Symbol: NTF3
• Synonyms: NTF3; NT3; HDNF; NGF2; NGF-2; MGC129711; NT-3; OTTHUMP00000239132; OTTHUMP00000239133; neurotrophic factor; nerve growth factor 2; neurotrophin 3; Nerve growth factor 2; Neurotrophic factor; neurotrophin-3
• Gene ID: 4908
• mRNA Refseq: NM_002527
• Protein Refseq: NP_002518
• MIM: 162660
• UniProt ID: P20783
• Chromosome Location: 12p13
• Function: growth factor activity; nerve growth factor binding; neurotrophin receptor binding; protein binding; receptor binding