Recombinant Human NEK2, GST-tagged, Active

• Cat.No.: NEK2-368H
• Product Overview: Recombinant full-length human NEK2 was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=66 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: NEK 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2. NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: NEK2 NIMA (never in mitosis gene a)-related kinase 2 [ Homo sapiens ]
• Synonyms: NEK2; NIMA (never in mitosis gene a)-related kinase 2; NLK1; NEK2A; HsPK21; NIMA-related kinase 2; EC 2.7.11.1
• Gene ID: 4751
• mRNA Refseq: NM_002497
• Protein Refseq: NP_002488
• MIM: 604043
• UniProt ID: P51955
• Chromosome Location: 1q32.2-q41
• Pathway: Cell Cycle, Mitotic
• Function: ATP binding; magnesium ion binding; transferase activity; nucleotide binding; protein phosphatase binding; protein serine/threonine kinase activity