Recombinant Human MTHFS protein, His-tagged

• Cat.No.: MTHFS-3464H
• Product Overview: Recombinant Human MTHFS(1-203aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-203 a.a.
• Description: MTHFS, also known as 5-formyltetrahydrofolate cyclo-ligase, is a cytosolic protein involved in the formate metabolic process. MTHFS, with a magnesium cofactor, catalyzes the ATP-dependent reaction that reduces 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate(MTHF). MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. MTHF is also a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD).
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl
• Molecular Mass: 25.4kDa (223aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHIFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRCMAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKDLQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STA
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MTHFS 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) [ Homo sapiens ]
• Official Symbol: MTHFS
• Synonyms: MTHFS; 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase); 5-formyltetrahydrofolate cyclo-ligase; HsT19268; methenyl-THF synthetase; FLJ30410
• Gene ID: 10588
• mRNA Refseq: NM_006441
• Protein Refseq: NP_006432
• MIM: 604197
• UniProt ID: P49914
• Chromosome Location: 15q24.3
• Pathway: Metabolic pathways, organism-specific biosystem; One Carbon Metabolism, organism-specific biosystem; One carbon pool by folate, organism-specific biosystem; One carbon pool by folate, conserved biosystem
• Function: 5-formyltetrahydrofolate cyclo-ligase activity; 5-formyltetrahydrofolate cyclo-ligase activity; ATP binding; folic acid binding; ligase activity; nucleotide binding