Recombinant Human MTHFR 293 Cell Lysate

• Cat.No.: MTHFR-4081HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for 5,10-methylenetetrahydrofolate reductase (NADPH) (MTHFR) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MTHFR methylenetetrahydrofolate reductase (NAD(P)H) [ Homo sapiens ]
• Official Symbol: MTHFR
• Synonyms: MTHFR; methylenetetrahydrofolate reductase (NAD(P)H); 5,10 methylenetetrahydrofolate reductase (NADPH); methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase (NADPH)
• Gene ID: 4524
• mRNA Refseq: NM_005957
• Protein Refseq: NP_005948
• MIM: 607093
• UniProt ID: P42898
• Chromosome Location: 1p36.3
• Pathway: Fluoropyrimidine Activity, organism-specific biosystem; Folate Metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of folate and pterines, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem
• Function: methylenetetrahydrofolate reductase (NADPH) activity; methylenetetrahydrofolate reductase (NADPH) activity; modified amino acid binding; oxidoreductase activity