Recombinant Human MTHFR protein, His & T7-tagged

• Cat.No.: MTHFR-1536H
• Product Overview: Recombinant Human MTHFR aa. (Met1~Arg325 (Accession # P42898）) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Arg325
• Description: The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.8kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 5.5

Gene Information

• Gene Name: MTHFR methylenetetrahydrofolate reductase [ Homo sapiens (human) ]
• Official Symbol: MTHFR
• Synonyms: MTHFR; methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase (NADPH); methylenetetrahydrofolate reductase (NAD(P)H)
• Gene ID: 4524
• mRNA Refseq: NM_001330358.1
• Protein Refseq: NP_001317287.1
• UniProt ID: P42898