Recombinant Human MS4A1 full length protein, His-tagged(Nanodisc)

• Cat.No.: MS4A1-3929H
• Product Overview: Recombinant Human MS4A1 full length protein(NP_068769.2)(Met 1 - Pro 297), fused to His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met 1 - Pro 297
• Description: B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. The protein has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. CD20 / MS4A1 is the target of the monoclonal antibodies (mAb) rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen.
• Form: Delivered as bulk protein in a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Molecular Mass: The CD20 carries a polyhistidine tag at the C-terminus with calculated MW of 35.2 kDa and migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein（MSP1D1）has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE) .
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: This product is stable after storage at:
  The product MUST be stored at -70°C or lower upon receipt;
  -70°C for 3 months under sterile conditions.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MS4A1 membrane-spanning 4-domains, subfamily A, member 1 [ Homo sapiens ]
• Official Symbol: MS4A1
• Synonyms: MS4A1; CD20; MS4A-1
• Gene ID: 931
• mRNA Refseq: NM_021950.3
• Protein Refseq: NP_068769.2
• MIM: 112210
• UniProt ID: P11836