Recombinant Human MMP9 protein, His-tagged

• Cat.No.: MMP9-811H
• Product Overview: Recombinant Human MMP9 protein(NP_004985. 2) (Met 1-Asp 707) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• ProteinLength: 1-707 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >800 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by APMA for an activated form)
• Molecular Mass: The recombinant human MMP9 consisting of 699 amino acids has a predicted molecular mass of 77.7 kDa. The apparent molecular mass of rhMMP9 is approximately 80-95 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) [ Homo sapiens ]
• Official Symbol: MMP9
• Synonyms: MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); CLG4B, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase), matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); GELB; CLG4B; MMP-9; MANDP2
• Gene ID: 4318
• mRNA Refseq: NM_004994
• Protein Refseq: NP_004985
• MIM: 120361
• UniProt ID: P14780