Recombinant Rat Mmp9 protein(Met1-Pro708), His-tagged

• Cat.No.: Mmp9-1195R
• Product Overview: Recombinant Rat MMP9 (EDL96479.1) (Met 1-Pro 708) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-708 a.a.
• Form: Lyophilized from sterile 50mM MES, 100mM NaCl, 1mM CaCl2, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1000 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by APMA for an activated form).
• Molecular Mass: The secreted recombinant rat MMP9 comprises 700 amino acids and predicts a molecular mass of 77.8 kDa. The apparent molecular mass of the rat MMP9 is approximately 100-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Mmp9 matrix metallopeptidase 9 [ Rattus norvegicus ]
• Official Symbol: Mmp9
• Synonyms: MMP9; matrix metallopeptidase 9; matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)
• Gene ID: 81687
• mRNA Refseq: NM_031055
• Protein Refseq: NP_112317