Recombinant Human METAP2 Protein, His&SUMO-tagged

• Cat.No.: METAP2-1109H
• Product Overview: Recombinant Human METAP2 Protein (Ala2-Tyr478) with His&SUMO tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&SUMO
• Protein Length: Ala2-Tyr478
• Description: The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 66.5 kDa; Accurate Molecular Mass: 90/33 kDa
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: METAP2 methionyl aminopeptidase 2 [ Homo sapiens (human) ]
• Official Symbol: METAP2
• Synonyms: METAP2; methionyl aminopeptidase 2; methionine aminopeptidase 2; MAP2; MNPEP; p67; Peptidase M; MAP 2; metAP 2; peptidase M 2; eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; p67eIF2
• Gene ID: 10988
• mRNA Refseq: NM_006838
• Protein Refseq: NP_006829
• MIM: 601870
• UniProt ID: P50579